Journal: Nature Communications

Article Title: An anionic, endosome-escaping polymer to potentiate intracellular delivery of cationic peptides, biomacromolecules, and nanoparticles

doi: 10.1038/s41467-019-12906-y

Figure Lengend Snippet: PPAA pretreatment enhances cationic nanoparticle uptake and bioactivity. a Schematic representation of cationic, amine-surface modified 200 nm polystyrene nanoparticles, synthesis of Cy5-labeled DNA-loaded D-DPB polymeric micellar nanoparticles, and sequential delivery of these nanoparticle formulations following PPAA pretreatment. The effects of dose-dependent PPAA pretreatment on the uptake of b cationic polysterene nanoparticles and c Cy5-labeled DNA-loaded D-DPB nanoparticles in A7r5 cells; * p < 0.05, vs. treatment with nanoparticles alone (i.e., 0 nM PPAA). d The effects of dose-dependent PPAA pretreatment on luciferase gene silencing in luciferase expressing A7r5s; * p < 0.05, ** p < 0.01, vs. D-DPB nanoparticles loaded with scrambled siRNA (scramble). For b – d , statistical analyses were performed with a one-way ANOVA followed by a Tukey’s post hoc test. Data are presented as means ± SEM. e PPAA dose-dependent effects on uptake vs. bioactivity

Article Snippet: Cell cultures were maintained on a 75 cm 2 polystyrene tissue culture flasks (T75; BD Falcon) and culture media was replaced every other day.

Techniques: Modification, Labeling, Luciferase, Expressing

Journal: STAR Protocols

Article Title: Protocol for functional characterization of endoreduplicated murine trophoblast cells

doi: 10.1016/j.xpro.2022.101573

Figure Lengend Snippet:

Article Snippet: T-75 tissue culture flask , Corning , 156499.

Techniques: Affinity Purification, Recombinant, Reverse Transcription, Protease Inhibitor, Microscopy, Membrane, Stripping Membranes, Spectrophotometry, Real-time Polymerase Chain Reaction, Flow Cytometry